How Long Can Breast Milk Sit Out at Room Temperature?

Information and Tips for Freshly Pumped, Refrigerated, and Frozen Breast Milk

How Long Can Breast Milk Sit Out at Room Temperature?

For healthy full-term infants, here are the guidelines for keeping breast milk out at room temperature.

  • Freshly pumped breast milk can stay out at room temperature (Up To 77 degrees F or 25 degrees C) for approximately 5 to 8 hours.
  • Refrigerated breast milk can sit out at room temperature for up to 4 hours.
  • Frozen breast milk that has been thawed in the refrigerator, but NOT yet warmed up can stay at room temperature for up to 4 hours.
  • Frozen breast milk taken directly from the freezer should NOT be stored or defrosted at room temperature.
  • Frozen breast milk that has been defrosted AND warmed up should be used immediately or placed in the refrigerator. It should NOT be stored at room temperature for any length of time.

Breast Milk and the Growth of Bacteria

Bacteria are all around. They are on your hands, on the skin around your breasts, and on the parts of your breast pump. When you pump your breast milk, some of that bacteria gets into the milk. But, don't worry, when you store your breast milk safely, this small amount of bacteria will not harm a healthy, full-term child.

Breast milk contains antibacterial and immune properties that can prevent bacteria from growing inside of it for many hours. However, the longer it is left out, the more time the bacteria has to multiply. Temperature also plays a significant role in the growth of bacteria. The higher the temperature of the room, the faster the bacteria can grow. So, to be safe, you should ideally use room temperature breast milk within 4 to 5 hours. But, if necessary, you could extend that time up to about 8 hours (preferably in a cooler room). After sitting out at room temperature for 8 hours, the bacteria can grow to unsafe levels.

While some sources state that due to the antibacterial properties found in human milk, it can remain at room temperature for as long as 10 to 12 hours, that is generally not the accepted recommendation.

The Centers for Disease Control and Prevention (CDC) and the Academy of Breastfeeding Medicine (ABM) recommend that breast milk should not stay out at room temperature for longer than 6 to 8 hours. The American Academy of Pediatrics (AAP) recommends no longer than 4 hours.

Storing Breast Milk for Premature Babies or Babies With Health Issues

These guidelines do not apply to premature infants or children that do not have healthy immune systems. The bacterial growth that occurs in breast milk that is left out at room temperature can be dangerous to children who at a higher risk of infection. In general, breastmilk pumped for premature or hospitalized infants should be used within one hour or refrigerated. Discuss the recommended storage guidelines for your situation with your child's health care providers.

Tips for Storing Breast Milk at Room Temperature

  • The temperature of the room should not be over 77 degrees F or 25 degrees C. The cooler the room, the better.
  • If the room temperature is greater than 77 degrees F (25 degrees C), do not let your breast milk sit out all. Place it in the refrigerator or an insulated cooler with ice packs immediately.
  • Once you collect your breast milk in the storage container, put a top or cap onto the breast milk storage bottle or seal the storage bag.
  • Place a cold towel over the storage container to keep the milk cooler.
  • Keep your breast milk away heat, windows, or areas that expose it to direct sunlight.
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Article Sources

  • Academy of Breastfeeding Medicine Protocol Committee. ABM clinical protocol# 8: Human milk storage information for home use for full-term infants. Original protocol March 2004; revision# 1 March 2010. Breastfeeding Medicine. 2010;5(3).
  • American Academy of Pediatrics. Breastfeeding Initiatives. FAQs.
  • Centers For Disease Control and Prevention. Proper Handling and Storage of Human Milk. 2010.
  • Lawrence, Ruth A., MD, Lawrence, Robert M., MD. Breastfeeding A Guide For The Medical Profession Eighth Edition. Elsevier Health Sciences. 2015.
  • Riordan, J., and Wambach, K. Breastfeeding and Human Lactation Fourth Edition. Jones and Bartlett Learning. 2014.